| Identifikační kód |
RIV/68081707:_____/21:00554407 |
| Název v anglickém jazyce |
A simple electroanalysis of polyG RNA in mixtures with 3',5'-cyclic guanosine monophosphate achieved by selective desorption of the monomers from the electrode surface |
| Druh |
J - Recenzovaný odborný článek (Jimp, Jsc a Jost) |
| Poddruh |
J/A - Článek v odborném periodiku je obsažen v databázi Web of Science společností Thomson Reuters s příznakem „Article“, „Review“ nebo „Letter“ (Jimp) |
| Jazyk |
eng - angličtina |
| Vědní obor |
10405 - Electrochemistry (dry cells, batteries, fuel cells, corrosion metals, electrolysis) |
| Rok uplatnění |
2021 |
| Kód důvěrnosti údajů |
S - Úplné a pravdivé údaje o výsledku nepodléhající ochraně podle zvláštních právních předpisů. |
| Počet výskytů výsledku |
4 |
| Popis výsledku v anglickém jazyce |
Previously it has been shown that cyclic nucleoside monophosphates can spontaneously polymerize to form RNA oligonucleotides under conditions simulating prebiotic conditions on Archean Earth. The most efficient polymerization was documented with 3',5'-cyclic guanosine monophosphate (cGMP). In this work a method for fast detection of short polyG RNAs present in a large overabundance of cGMP, modeling conditions in the non-enzymatic nucleotide polymerization mixtures, is presented. The method is based on electrochemical measurements of guanine (G) oxidation signals yielded by RNA oligomers adsorbed onto the surface of a pyrolytic graphite electrode (PGE). To avoid false positive results arising from the G oxidation signals due to coadsorbed cGMP, a method for selective removal of the monomers from the electrode surface has been devised. In the first step, both cGMP and RNAs are co-adsorbed onto the PGE surface. In the second step, the cGMP is selectively desorbed using treatments in solutions of different tested surfactants (SDS, Tween 20 or Triton X100), or by washing in deionized water at elevated temperature. We show that this new approach is suitable for selective analysis of products of polymerization reactions from mixtures of their building blocks. |
| Klíčová slova oddělená středníkem |
electrochemical oxidation;nucleic-acids;oligonucleotides;dna;voltammetry |
| Stránka www, na které se nachází výsledek |
https://www.sciencedirect.com/science/article/pii/S1572665721007992?via%3Dihub |
| DOI výsledku |
https://doi.org/10.1016/j.jelechem.2021.115773 |
| Odkaz na údaje z výzkumu |
- |